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Impact of amino acids and reducing agents on toxin expression. Toxin yield (U/ml; left scale and squares) and cell yield (OD600; right scale and circles) in C. difficile VPI 10463 grown for 48 h in PY with different concentrations of various amino acids, thioglycolate, and cysteine derivatives added. Where indicated, the compound inhibited growth or was not soluble. The mean and standard error of three experiments are shown.
