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Impact of butyric acid or butanol on toxin yield (U/ml) in C. difficile. Strain VPI 10463 was inoculated into (A) different media (PY, PY supplemented with 10 mM cysteine or with 0.9% glucose [PYG], and SDM), with or without 30 mM butyric acid added, and (B) PY supplemented with different concentrations of butanol. Toxin yield was measured after 48 h of growth. The mean and standard error of three experiments are shown.
