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. 2023 Mar 7;11(5):629–645. doi: 10.1158/2326-6066.CIR-22-0655

Figure 2.

Figure 2. Distinct immune profile evolution of EGFR-WT and EGFRvIII GBMs during progression. A, BLI of GBMs at early- and late-stage tumor progression. n = 6 each EGFR-WT and EGFRvIII early and late stage. B, Relative number of EGFR+ GBM cells during early- and late-stage tumor progression. n = 4,6 and 4,6 for EGFR-WT early and late stage and EGFRvIII early and late stage, respectively. C–I, Flow cytometry longitudinal assessment of the indicated cell types during early- and late-stage tumor progression. J, Suppression of proliferation of T cells by MDSCs isolated from EGFR-WT and EGFRvIII GBM bearing mice. n = 4 for each control, EGFR-WT, and EGFRvIII mice. K, Ratio of CD8:PMN-MDSC from early- and late-stage GBMs. L, and M, Flow cytometry analysis of relative CD8+ and CD4+ TEM and TCM cell subset from GBM (L) and TDLNs (M) tissues of early- and late-stage GBMs. All data are mean ± SEM of biological replicates (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; unpaired t test, two-tailed).

Distinct immune profile evolution of EGFR-WT and EGFRvIII GBMs during progression. A, BLI of GBMs at early- and late-stage tumor progression. n = 6 each EGFR-WT and EGFRvIII early and late stage. B, Relative number of EGFR+ GBM cells during early- and late-stage tumor progression. n = 4,6 and 4,6 for EGFR-WT early and late stage and EGFRvIII early and late stage, respectively. C–I, Flow cytometry longitudinal assessment of the indicated cell types during early- and late-stage tumor progression. J, Suppression of proliferation of T cells by MDSCs isolated from EGFR-WT and EGFRvIII GBM bearing mice. n = 4 for each control, EGFR-WT, and EGFRvIII mice. K, Ratio of CD8:PMN-MDSC from early- and late-stage GBMs. L, and M, Flow cytometry analysis of relative CD8+ and CD4+ TEM and TCM cell subset from GBM (L) and TDLNs (M) tissues of early- and late-stage GBMs. All data are mean ± SEM of biological replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; unpaired t test, two-tailed.