Extended Data Fig. 5 ∣. Target identification and resistance mutations.

BamA crystal structure (green) with labeled resistance mutation sites identified in this paper: (A) front view of lateral gate and (B) view of the barrel lumen from the periplasmic side (underside). Sites identified which gave resistance to both compounds are labeled in blue, and a mutation site which gives resistance to only dynobactin A is labeled in magenta. (C) Table listing MICs for bacteria from the previously described darobactin-resistance evolution experiment¹⁵, the isolated dynobactin-resistant mutants from this study, and other E. coli strains with outer membrane deficiencies (that is porin or efflux knockouts).