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Performance efficiency of qRT-PCR assay for SARS-CoV-2 and Phi6 gene targets
| Gene | Efficiency (%) | Slope | Y-Intercept | R2 value | Assay limit of detection (copies/5 μL of RNA template) |
|---|---|---|---|---|---|
| N1 gene | 87.05 | − 3.677 | 43.719 | 0.988 | 300 |
| ORF1b-nsp14gene | 71.34 | − 4.276 | 45.673 | 0.991 | 300 |
| RdRp gene | 75.52 | − 4.093 | 46.084 | 0.983 | 300 |
| Phi6 | 73.248 | − 4.19 | 43.053 | 0.999 | 80 |
