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. 2023 May 3;16:46. doi: 10.1186/s13045-023-01442-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Cell fusion in the bone marrow can generate myeloid-like tumor hybrid cells. a Schematic illustration for identifying hybrid tumor cells. b Experimental schema for isolating hybrid tumor cells from the bone marrow of mice with bone metastasis. c Fluorescence microscope images of hybrid tumor cells. d Flow cytometry analysis of parental RM1 (mCherry-NLS) and hybrid tumor cells (scale bar: 50 µm). e Agarose gel electrophoresis of PCR products for EGFP and mCherry primers in RM1 cells, bone marrow (EGFP mouse), and tumor hybrid cells. f Karyotype analysis of RM1 cells and tumor hybrid cells. g Chromosome numbers in parental RM1 and hybrid tumor cells. h Volcano plot showing the differentially expressed genes between parental RM1 and hybrid tumor cells. i, j GO enrichment analysis of up-regulated genes (i) or proteins (j) in tumor hybrid cells. ***P < 0.001