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. 2023 May 2;11:96. doi: 10.1186/s40168-023-01546-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MWD induces intestinal microbiota and metabolite shifts in the offspring. Feces from the W-N and N-N group mice were collected for 16s rDNA analysis and untargeted metabolomics analysis. a, b α-diversity analysis of gut microbes reflected by Shannon and Chao indices. c Scatter plots of weighted PCoA for the microbial composition. d Relative abundance of gut microbiota at the phylum level. e KEGG pathway enrichment analyses of the upregulated differential metabolites in the W-N group. The dot size represents the number of differential metabolites, and the dot color represents the corresponding p value. f Scatter plot showing differential metabolites in the W-N group versus the N-N group. Differential metabolites were plotted based on their expression levels (log10 intensity). Red and green dots represented up- and downregulated genes, respectively. Differential bile acids were indicated. g Top 10 differential metabolites in the W-N group versus the N-N group. The y axis shows the name of metabolites, and the x-axis shows the fold change of metabolites