FIG. 5.

Photomicrographs of the intimin mutant and parental wild-type strain in a rhodamine-phalloidin assay for FAS in Caco-2 cells. (A) Caco-2 cells infected by bacteria as described in the Fig. 4 legend are shown as follows: in fluorescent views after treatment with anti-O157 LPS rabbit antibody followed by anti-rabbit goat antibody conjugated with fluorescein isothiocyanate (Bacteria), in fluorescent views of actin stained by rhodamine-phalloidin (Actin), and in a superimposed view of bacteria (green) and actin (red) (Super impose). Strains and incubation times are indicated at the left of photos. (B) Quantitative FAS assay of the O157Sakai wild type (WT) and the intimin mutant (Δeae), derived from the experiments whose results are shown in panel A.