TABLE 2.
Products encoded by the gene witha or in the vicinity of mini-Tn5Km2
| Strain | Gene product | Site (min) on E. coli K-12 genomeb | Accession no. or reference |
|---|---|---|---|
| A3-H2 | Hypothetical 17.8-kDa protein (yfjG) | 59.2 | P52121 (1) |
| C6-E9 | Putative membrane protein | – | AE000234 |
| C6-F9 | Putative membrane protein | – | AE000234 |
| C6-H10 | Putative membrane protein | – | AE000234 |
| C7-A10 | Putative membrane protein | – | AE000234 |
| C8-A2 | Hypothetical 8-membrane-spanning 37.6-kDa protein (yiaH) | 80.2 | P37669 |
| F4-H4 | Putative NAGC-like transcriptional regulator (yphHI) | 57.8 | AE000341 |
| ABC transporter-periplasmic binding protein YPHF precursor | P77269 | ||
| Hypothetical 127.3-kDa protein | P76585 | ||
| Serine hydroxy transferase (glyA) | 38 | ||
| F5-F4 | Putative potassium channel (kch) | 28.3 | P31069 (32) |
| F6-B9 | Hypothetical 10-membrane-spanning 58.4-kDa protein | 27.2 | P40877 |
| F6-D11 | Hypothetical 40.2-kDa protein | 64.7 | Q46803 |
| Hypothetical sigma 54-dependent transcriptional regulator | Q46802 | ||
| G2-B12 | Putative adherence factor (efa1) | – | AF159462 (34) |
| G4-B6 | Arsenite (efflux) pump membrane protein (arsF) | 78.59 | 7 |
| Origopeptidase A (prlC) | 3 | ||
| G4-B12 | Hypothetical 27.7-kDa protein | 14.6 | P77627 |
| G4-C10 | (orf3 of EPEC pathogenicity island LIM) | – | 46 |
| G4-E10 | Lysine-specific permease (lysP) | 48.5 | 43 |
| Hypothetical transcriptional regulator | P32484 | ||
| H7-C4 | Hypothetical 10-membrane-spanning 43.6-kDa protein | 87.5 | P32135 |
Products encoded by the gene with mini-Tn5Km2 are in bold type.
The insertion sites placed in the DNA sequences specific to the O157Sakai genome sequence are indicated by a dash (–). The sites of C6-E9, C6-F9, C6-H10, and C7-A10 were in the same ∼3-kb regions encoding an open reading frame partially homologous to the b1372 gene, which encodes a putative membrane protein of 1,122 amino acids of E. coli K-12. F6-D11 was placed in a region containing genes encoding a hypothetical sigma 54-dependent transcriptional regulator and a hypothetical 40.2-kDa protein homologous to ornithine carbamoyltransferase. G4-C10 was placed in orf3 in a region homologous to the EPEC pathogenicity island named LIM, encoding a chaperon-like protein involved in the stable expression of BfpA and intimin (47).