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. Author manuscript; available in PMC: 2023 May 3.
Published in final edited form as: Plast Reconstr Surg. 2022 Oct 7;151(1):72e–84e. doi: 10.1097/PRS.0000000000009798

Figure 3. Emulsification and Micronization Device (EMD) results for human LA.

Figure 3.

Human LA (N = 5) was mechanically processed by manually shuffling between two syringes (nanofat, NF) or using the EMD with 30 device passes. Unprocessed LA is indicated as macrofat (MF). All samples were digested with collagenase prior to cell analysis. (A) Nucleated cell counts decreased by ~half for NF and all device-processed conditions. (B) Nucleated cell viability remained at ~90% for all conditions. (C) Stem and progenitor cells were identified by flow cytometry, and the relative population percentage was calculated and normalized to MF (value=1). Mechanical processing enriched stem/progenitor cell types of interest, often by 2- to 3-fold compared to MF, while the EMD provided similar or improved results. Error bars represent standard error from at least three independent experiments.