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Mapping of the transcription start site of P2. Total RNA and a 5′ end-labeled primer PrEx2 were used in primer extension reactions. The resulting cDNAs and sequencing reactions from pBlue1-1, harboring the htrA regulatory region, were loaded on an 8% acrylamide gel. The start of transcription was mapped to A165, and the complementary thymine residue on the other strand is indicated (arrow). Lanes: 1, B. henselae htrA; 2, B. henselae groEL control.
