FIG. 5.

Thermal induction of htrA promoter regions in the reverse orientation in B. henselae 882str by flow cytometry. Bacteria harboring pSIR14, pSIR15, or pSIR16 were grown at 27 or 37°C. Bacterial growth was harvested after two days, resuspended in PBS, and analyzed in the FACSCalibur. B. henselae 882str was electrotransformed with the following plasmids: pANT3 (A), pSIR14 (harboring P1R) (B), pSIR15 (harboring P2R) (C), and pSIR16 (harboring P1R-P2R) (D). Arrows indicate the mean fluorescence channel (X) at 27 and 37°C. One representative set of histograms is displayed.