TABLE 1.
Description of vectors, resulting constructs, and E. coli strains
| Plasmid or strain | Application or genotype | Source or reference |
|---|---|---|
| Plasmids | ||
| pBlue1-1 | pBluescript/htrA regulatory region | 3 |
| pANT3 | Promoterless gfpmut3; used to evaluate htrA promoter activity in B. henselae | 26 |
| pSIR11 | pANT3/B. henselae htrA P1 | This work |
| pSIR12 | pANT3/B. henselae htrA P2 | This work |
| pSIR13 | pANT3/B. henselae htrA P1-P2 | This work |
| pSIR14 | pANT3/B. henselae htrA P1R | This work |
| pSIR15 | pANT3/B. henselae htrA P2R | This work |
| pSIR16 | pANT3/B. henselae htrA P1R-P2R | This work |
| E. coli strains | ||
| JM109 | F′ traD36 lacIq Δ(lacZ)M15 proA+ B+/e14−(McrA−) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17(rK− mK+ mK+) relA1 supE44 recA1 | |
| DH12S | mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL/ F′ proAB+ lacIqZΔM15 |