TABLE 2.
Primer pairs used for the PCR amplification of htrAa promoter regions and the kanamycin resistance gene
| Region amplified | Primer | Sequence |
|---|---|---|
| P1 PCR | P1 | 5′ AAAAGGATCCTACTTTGAATGCTAG 3′ |
| P1A | 5′ AAAATCTAGATTTTTTCCTGTAACA 3′ | |
| P2 PCR | P2 | 5′ AAAAGGATCCTCATCAGGATGTATT 3′ |
| P2A | 5′ AAAATCTAGAAACAAACCTTACCGT 3′ | |
| P1Rb PCR | P1R | 5′ AAAATCTAGATACTTTGAATGCTAG 3′ |
| P1AR | 5′ AAAAGGATCCTTTTTTCCTGTAACA 3′ | |
| P2Rb PCR | P2R | 5′ AAAATCTAGATCATCAGGATGTATT 3′ |
| P2AR | 5′ AAAAGGATCCAACAAACCTTACCGT 3′ | |
| Kanamycin resistance gene | TnKn903-1 | 5′ CCGATGCGCCAGAGTTGTTTCTGAA 3′ |
| TnKn903-2 | 5′ ACCTATTAATTTCCCCTCGTCAAAA 3′ |
a
Primer pairs P1-P2A and P1R-P2AR were used to amplify the tandem promoter regions P1-P2 and P1R-P2R, respectively.
b
R, used to clone promoter regions in the reverse orientation in pANT3.