Figure S6.

High-resolution structures of CAND1-CUL1-RBX1 and CAND1β-hairpin++-SCF^SKP2, related to Figure 6

(A) Cryo-EM data for CAND1-CUL1-RBX1 showed only the CAND1 engaged conformation. CAND1-CUL1-RBX1 interface density is shown on the left, and right in cartoon shows the CUL1-CAND1 β-hairpin interface.

(B) Cryo-EM density for CAND1β-hairpin++-SCF^SKP2 shows CAND1 rolling-2/SCF engaged and CAND1 partly engaged/SCF partly rocked conformations. CAND1-CUL1-RBX1 interface density is shown on the left, and the right shows in cartoon the CUL1-SKP1 interface. These maps superimpose with those for CAND1Δβ-hairpin-SCF^SKP2 shown in Figure S5C. Dotted oval in bottom right panel highlights where density is missing for CAND1 β-hairpin++ mutant.

(C) Representative gels of the in vitro reconstitution assay shown in Figure 6F.