Figure S4.
Fraction of Rbp4-Cre ROIs with calcium events, as well as the frequency, length, and size of calcium events all vary across embryonic days, related to Figure 3
(A) Spontaneous calcium activity recorded from a single Rbp4-Cre neuron, showing three detected events, with event properties quantified for each event.
(B–I) Quantification of event properties for Rbp4-Cre somas (B, D, F, and H) and neurites (C, E, G, and I). On each embryonic day from E13.5 to E18.5, these event statistics include the event rate (B and C), fraction of Rbp4-Cre ROIs showing spontaneous calcium events in each 10-min recording (D and E) and, for each detected calcium event, the size (F and G) and length (H and I) of the event. Circles: event property of each ROI; box (25–75 percentile) and whisker (5–95 percentile); white line: median. Inset: mean ± SEM. (B – E) n = number of Rbp4-Cre ROIs (somas or neurites) recorded from 3 (E13.5), 9 (E14.5), 5 (E15.5), 4 (E16.5), 5 (E17.5), and 6 (E18.5) embryos. (F – I) n = number of detected calcium events. Probability: Wilcoxon rank-sum test. Scale bar: 5s (A), 2⋅10−3 Hz (inset, B), 4⋅10−3 Hz (inset, C), 2 ΔF/F (inset, F), 2 ΔF/F (inset, G), 10s (inset, H), 10s (inset, I).