Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 27;186(9):1930–1949.e31. doi: 10.1016/j.cell.2023.03.025

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Author(s)

This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

PMC Copyright notice

Figure S6 — All Rbp4-Cre neurons display voltage gated sodium channels dependent active conductances, at both E14.5 and E18.5, related to Figure 4

(A–D) All Rbp4-Cre neurons (A, C), but not all nearby unlabeled cells (B, D), display active conductances, at both E14.5, and E18.5. Voltage responses (top) of an Rbp4-Cre neuron and a nearby unlabeled cell at E14.5 (A and B) and E18.5 (C and D) to graded intracellular current injections (middle), recorded in current clamp mode. The voltage responses to current are quantified in both current-voltage curves and the peak voltage compared to the steady-state voltage (as in Figure 4C) for both Rbp4-Cre neurons (C) and nearby unlabeled neurons (D), to visualize any nonlinearity in the peak voltage response. The line type labels the embryonic layer in which each recorded neuron was found (E14.5: Dotted: superficial layer, solid: deep layer; E18.5: Solid: intermediate layer). Due to visibility at E18.5, it was not possible to record from deep layer neurons. n = number of neurons.

(E) Left: schematic of para-uterine imaging combined with patch clamp recording to perform in vivo two-photon targeted electrophysiology from Rbp4-Cre neurons together with application of TTX. Right: Voltage responses of an Rbp4-Cre neuron (at E14.5 (top) and E18.5 (bottom)) to graded intracellular current injections, recorded in current clamp mode before (left, pre) and after (right, post) application of TTX. Scale bar: 10 mV (top, A), 50 ms and 20 pA (middle, A), 10 mV (top, B), 50 ms and 20 pA (middle, B), 10 mV (top, C), 50 ms and 20 pA (middle, C), 10 mV (top, D), 50 ms and 20 pA (middle, D), 20 mV (top, E), 10 ms and 40 pA (bottom, E).