TABLE 2.

Oligonucleotide primers used in RT-PCR and in situ hybridization analyses

Oligonucleotide primer	Sequence	Position in E. coli rRNA gene sequence
R1	5′ AGAGTTTGATCCTGGCTCAG 3′	8–27
R2	5′ ACTGCTGCCTCCCGTAGGAG 3′	339–358
ISH1	5′ ATTCCCCACTGCTGCCTCCCGTAGGAGT 3′	338–365
ISH2	5′ GACTTGACGTCATCCCCACCTTCCTCC 3′	1175–1300
ISH3	5′ CGGGCGGTGTGTACAAGGCCCGGGAACG 3′	1378–1405
B40F	5′ GTTTTCCCAGTCACGAC 3′	NAa
B40R	5′ AGCGGATAACAATTTCACACAGGA 3′	NA

^aNA, not applicable.