Extended Data Fig. 4. Mutations in Rht1, ZnF, and EamA generated by CRISPR-Cas9 gene editing.

a–c, Fielder plants with mutations in ZnF (a), Rht-B1b (b), or EamA (c). d, Fielder plants with single or double mutations in ZnF-B and Rht-B1b generated from a single gene-editing experiment. The symbols ‘+’ and ‘−’ indicate the insertion and deletion of nucleotide, respectively, and the base numbers of insertion/deletion (bp) were shown on the right. The sgRNA target sequences were marked by pink boxes, and the PAM motifs were highlighted in red letters.