Fig. 12.

COX7RP expression levels regulate formation of mitochondrial supercomplexes in rat ventricular myocytes. A, B Transfection of rat ventricular cardiomyocytes with Ad-COX7RP-FLAG (male (M)) and with shRNA COX7RP (female (F)) alters expression levels of COX7RP protein 48 h after infection (10 MOI). Hsp60 was used as loading control. B Pooled data for (A), Mean ± SEM, N = 4 M and N = 5 F, *p < 0.05, Student’s t-test. C Representative BN-page experiments demonstrating that COX7RP overexpression increases formation of multimolecular ETC supercomplexes in Ms, while COX7RP shRNA decreases it in Fs. Antibodies used: Complex I—anti-NDUFA9; Complex III—anti-UQCRFS1; Complex IV—anti-COX IV; anti-GAPDH all from Abcam. Hsp60 was used as loading control (inset, Anti-Hsp60 from Cell Signaling). Supercomplexes (SCs) are indicated by black bars. D Pooled normalized optical density data for (C) mean ± SEM, N = 4 M and N = 5 F animals, *p < 0.05, Student’s t-test