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. 2023 Apr 13;33(5):372–388. doi: 10.1038/s41422-023-00790-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–d In BM chimera experiments, BM cells from Carinh^WT and Carinh^KO mice were transplanted into 1100 rad γ-ray irradiated recipient mice. Two months later, the DSS colitis model was induced (a). Colitis was monitored by body weight loss (b), colon shortening (c), and H&E staining of colon tissues (d). For H&E staining (d): left, representative pictures. Scale bar, 50 μm. Right, quantification of corresponding histology scores. 5 views per mice, 8 mice per group. BMT, bone marrow transplantation. BM cells from congenic mice were transplanted into 1100 rad γ-ray irradiated Carinh^WT and Carinh^KO recipient mice. Two months later, the DSS colitis model was induced (e). Colitis was monitored by body weight loss (f), colon shortening (g), and H&E staining of colon tissues (h). For H&E staining (h): left, representative pictures. Scale bars, 50 μm. Right, quantification of corresponding histology scores. 5 views per mice, WT to Carinh^WT n = 7 mice and WT to Carinh^KO n = 5 mice.PBS (Ctrl) or Clodronate liposomes (Clo.) or anti-CSF-1R antibodies were i.p. injected 2 days before (–2d) and 4 days after (+4d) 2% DSS administration (i). Colitis was monitored by body weight loss (j and l), colon shortening (k and m, representative pictures; n, statistics) and H&E staining of colon tissues (o, p). (o) Representative pictures. Scale bars, 50 μm. p Quantification of corresponding histology scores. 5 views per mice, 10 Carinh^WT (Ctrl) mice, 7 Carinh^KO (Ctrl) mice, 7 Carinh^WT (Clo.) mice, 6 Carinh^KO (Clo.) mice, 5 Carinh^WT (anti-CSF-1R) mice, and 6 Carinh^KO (anti-CSF-1R) mice. Body weight data in b, f, j and l are pooled from two independent experiments. Other data are representative of three independent experiments. Data represent means ± SEM. Body weight changes in b, f, j and l were analyzed by two-way ANOVA. Colon length (n) and histology scores (p) were analyzed by one-way ANOVA. Unpaired two-tailed Student’s t-tests were used for other analyses. **P < 0.01, ****P < 0.0001; ns, not significant.