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. 2000 Oct;68(10):6027–6033. doi: 10.1128/iai.68.10.6027-6033.2000

FIG. 4.

FIG. 4

FIG. 4

Detection of pMGA1.2 expression. (A) Northern analysis of 3 μg of total RNA from M. gallisepticum S6 cells and clones, described in the text, using a probe specific for the pMGA1.1 or pMGA1.2 gene as indicated. (B) Alignment of the DNA sequences of the 5′ region of the pMGA1.2 genes amplified from S6J and C20.2A cells. Dots represent identities to the S6J sequence, and dashes represent deletions. Arrows above the S6J sequence indicate the orientations and locations of the oligonucleotides [1.2(GAA)F and 1.2(GAA)R] used to amplify the PCR products.