Figure 2.

Intersectional targeting of c-Maf^EX and c-Maf^IN neurons

(A) Intersectional targeting strategy of c-MafEX neurons.

(B) Immunofluorescence staining of a transversal section of the lumbar spinal cord of c-MafEX mice injected in the spinal cord with rAAV9.CAG.C_on/D_on.eGFP, showing the overlap between eGFP⁺ and Lmx1b⁺ neurons. Scale bars, 50 μm.

(C) Quantification of the number of eGFP⁺ neurons positive for Lmx1b and Pax2 in (B) (n = 4, 853 eGFP⁺ neurons).

(D) Immunofluorescence staining of a lumbar DRG section of c-Maf^EX mice showing no expression of eGFP in sensory neurons following spinal cord injection. Scale bars, 50 μm.

(E) Localization of eGFP-labeled neurons relative to PKCγ immunoreactive cell layer (n = 4). Scale bars, 100 μm.

(F) Localization of eGFP-labeled neurons relative to CGRP, IB4, and VGlut1 immunoreactivity (n = 4). Scale bars, 100 μm.

(G) Morphology of sparsely labeled c-Maf^EX neurons. Coordinates are dorsal and ventral (d + v) and medial and lateral (m + l).

(H) Example traces recorded from c-Maf^EX neurons.

(I) Venn diagram indicating the proportion of firing patterns that have been observed in c-Maf^EX neurons (n = 22 cells).

(J) Intersectional targeting strategy of c-MafIN neurons.

(K) Immunofluorescence staining of a transversal section of the lumbar spinal cord of c-MafIN mice injected in the spinal cord with rAAV9.CAG.C_on/D_on.eGFP, showing the overlap between eGFP⁺ and Pax2⁺ neurons. Scale bars, 50 μm.

(L) Quantification of the number of eGFP⁺ neurons positive for pax2⁺ in (C) (n = 3, 611 neurons).

(M) Immunofluorescence staining on DRG sections showing no expression of the eGFP transgene in sensory neurons. Scale bars, 50 μm.

(N) Localization of eGFP-labeled neurons relative to PKCγ immunoreactive cell layer (n = 3). Scale bars, 100 μm.

(O) Localization of eGFP-labeled neurons relative to CGRP, IB4, and VGlut1 immunoreactivity (n = 3). Scale bars, 100 μm.

(P) Morphology of sparsely labeled c-Maf^IN neurons. Coordinates are rostral and caudal (r + c) and medial and lateral (m + l).

(Q) Example traces recorded from c-Maf^IN neurons.

(R) Venn diagram indicating the proportion of firing patterns that have been observed in c-Maf^IN neurons (n = 18 cells).

Error bars denote ±SEM. Scale bars, 50 μm (B and K) and 100 μm (D–F and M–O).