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. 2023 Mar 21;42(4):112295. doi: 10.1016/j.celrep.2023.112295

Figure 5.

Figure 5

Pharmacogenetic modulation of c-Maf spinal interneuron activity in chronic pain states

(A) DREADD expression was driven by injection of rAAV.EF1α.Con/Don.hM4Di (c-MafEX mice) or rAAV.EF1α.Con/Don.hM3Dq (c-MafIN mice) into the lumbar spinal cord of c-MafEX, c-MafIN, and control mice.

(B) Virus injection was followed by CCI to induce neuropathic pain.

(C–E) Responses to mechanical stimulation using the von Frey (C), pin prick (D), or light brush (E) tests before and after induction of neuropathic pain with CCI and silencing of c-MafEX neurons (c-MafEX: n = 8; control: n = 7; Table S1).

(F–H) Responses to mechanical stimulation using the von Frey (F), pin prick (G), or light brush (H) tests before and after induction of neuropathic pain with CCI and activation of c-MafIN neurons (c-MafIN: n = 8; control: n = 8; Table S1).

PWL, paw withdrawal latency; BL, baseline before injury; CCI, BL 7 days after chronic constriction injury and before CNO injection. 1 h to 24 h refers to time post CNO injection. Error bars denote ±SEM. Number of mice and statistics are shown in Table S1. In brief: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (ANOVA, followed by pairwise comparisons).