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Huh7 cells, without or with Flag‐tagged SIRT3 overexpression, were cultured in medium with light or heavy isotope, respectively. Immunoblot of Kla and SIRT3 in the total cell lysates that were isolated from the above Huh7 cells upon NaLa treatment (25 mM for 24 h). GAPDH was used as the loading control.
