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. 2023 Mar 10;24(5):e56052. doi: 10.15252/embr.202256052

Figure 2. Identification of SIRT3 delactylation targets in Huh7 cells.

Figure 2

  1. The schematic overview of the SILAC combined with HPLC‐MS/MS strategy to identify SIRT3 delactylation targets. Huh7 cells (without or with SIRT3 overexpression) were cultured in a medium with light or heavy isotopes, respectively. After the total cell lysates extraction, the isotopically labeled lysates were mixed and immunoprecipitated with anti‐lactyllysine antibody‐conjugated beads, and then were proceed to HPLC‐MS/MS analysis.
  2. The distribution of the number of lysine lactylation sites per protein.
  3. The pathway functional enrichment analysis of lysine lactylated proteins.