TABLE 1.

Sequences of primers used for multiplex polymerase chain reaction assays for extended spectrum β-lactamase genes, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022.

Primer	Sequence (5ʹ-3ʹ direction)	Amplicon size	Reference
bla-SHV.SE forward	ATGCGTTATATTCGCCTGTG	747 bp	Monstein et al.13
bla-SHV.AS reverse	TGCTTTGTTATTCGGGCCAA
TEM-164.SE forward	TCGCCGCATACACTATTCTCAGAATGA	445 bp
TEM-165.AS reverse	ACGCTCACCGGCTCCAGATTTAT
CTX-M-U1 forward	ATGTGCAGYACCAGTAARGTKATGGC	593 bp
CTX-M-U2 reverse	TGGGTRAARTARGTSACCAGAAYCAGCGG

Source: Adapted from Monstein HJ, Ostholm-Balkhed A, Nilsson MV, Nilsson M, Dornbusch K, Nilsson LE. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. APMIS. 2007;115(12):1400–1408. https://doi.org/10.1111/j.1600-0463.2007.00722.x

bp, base pairs.