TABLE 2.
Description of ceftriaxone-resistant GNB recovered for multiplex polymerase chain reaction amplification and detection of extended spectrum β-lactamase genes, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022.
| Variable | Frequency (n) | Percentage (%) |
|---|---|---|
| Ward/unit of isolation | ||
| Burn unit | 12 | 40.0 |
| NICU | 7 | 23.3 |
| PICU | 5 | 16.6 |
| Medical ward | 4 | 13.3 |
| AICU | 2 | 6.6 |
| Sample of origin | ||
| Pus or pus swab | 17 | 56.6 |
| Urine | 7 | 23.3 |
| Blood | 6 | 19.9 |
| Isolate name | ||
| E. coli | 13 | 43.3 |
| K. aerogenes | 7 | 23.3 |
| E. cloacae | 3 | 10.0 |
| Acinetobacter spp. | 3 | 10.0 |
| P. aeruginosa | 2 | 6.6 |
| K. oxytoca | 1 | 3.3 |
| A. hydrophilia | 1 | 3.3 |
| Disk combination method results | ||
| Positive | 30 | 100.0 |
| Negative | 0 | 0.0 |
| Multiplex PCR amplification results | ||
| Positive | 25 | 83.3 |
| Negative | 5 | 16.7 |
| Type of ESBL gene | ||
| blaCTX-M | 23 | 92.0 |
| blaTEM | 18 | 64.2 |
| blaSHV | 2 | 8.0 |
| Multiple ESBL genes | ||
| Yes | 18 | 72.0 |
| No | 1 | 28.0 |
| Type of multiple ESBL genes | ||
| blaCTX-M and blaTEM | 16 | 88.8 |
| blaCTX-M and blaSHV | 1 | 5.6 |
| blaCTX-M and blaTEM and blaSHV | 1 | 5.6 |
AICU, adult intensive care unit; NICU, neonatal intensive care unit; PICU, paediatric intensive care unit; ESBL, extended spectrum β-lactamase; PCR, polymerase chain reaction.