Figure 2 .

S100a9 rescues exhausted CD8 ⁺ T cells via the PI3K/AKT/mTOR signaling pathway

(A) Lymphocytes were isolated from MDS patient specimens and healthy control (HC) specimens ( n=4 LR-MDS, n=5 HR-MDS, n=3 HC and n=5 HR-MDS+rhsS100a9). Lymphocytes were treated with IL-2 (50 IU/mL) for 72 h and then cocultured with SKM-1 cells for 24 h. The cytotoxicity was measured by CCK8 assay. (B) SKM-1 cells and K562 cells were treated with rhS100a9 (20 μg/mL for SKM-1, 40 μg/mL for K562) for 72 h. PI3K, AKT and mTOR levels were measured by western blot analysis. Data are expressed as the mean±SD from three experiments. ** P<0.01.