FIG. 1.

Transfection of CHO cells with pExoT-HA. (A) CHO cells were cotransfected with 50 ng of pEGFP alone or with 300 ng of pExoT-HA. Twenty-four hours posttransfection, cells were fixed, stained with phalloidin-rhodamine, and visualized by fluorescence microscopy for GFP or rhodamine (phalloidin). (B) Lysates from CHO cells transfected with pEGFP (lane 1) or pEGFP and pExoT-HA (lane 2) were subjected to ECL Western blot analysis, using rabbit anti-HA IgG (upper section) or mouse anti-GFP IgG (lower section) as the primary antibody and HRP-conjugated goat anti-rabbit or mouse IgG as the secondary antibody. (C) CHO cells were transfected with the indicated amount of pExoT-HA and 50 ng of reporter plasmid (pEGFP) and, 24 h posttransfection, were scored for morphology. The percentages of rounded cells shown are averages from analysis of three fields of transfected cells (approximately 50 transfected cells per field).