Figure 5 .

Calycosin decreased the expressions of HuR and IGF2BP1 in breast cancer cells

(A) The potential targets of lncRNA HOTAIR were identified by bioinformatics analysis using StarBase ( https://starbase.sysu.edu.cn/starbase2/). (B) The targeted relationships between lncRNA HOTAIR, HuR and IGF2BP1 were verified by RNA binding protein immunoprecipitation in MDA-MB-231 and SK-BR-3 cells. IP/input% enrichment efficiency obtained by qRT-PCR (input stands for internal reference). The protein level (C) and transcription level (D) of HuR and IGF2BP1 were detected in MCF-7, T47D, MDA-MB-231 and SK-BR-3 cells after treatment with calycosin (0, 20, 40 and 80 μM). β-Actin and GAPDH were used as internal controls. Data are presented as the mean±SD ( n=3). * P<0.05, * * P<0.01 and *** P<0.001 compared with the control group.