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. 2023 Apr 9;13(7):2226–2240. doi: 10.7150/thno.81325

Figure 6.

Figure 6

Mechanism validation of photothermal therapy with PFIRM. (A) Immunofluorescent staining of CRT in tumor. Scale is 20 μm. (B-C) Flow cytometry analysis of the percentage of CD80+CD86+ DCs in draining lymph nodes (n=3). (D) Immunofluorescent staining of MHC-I and LAMP2 in tumor. Scale bar is 10 μm. (E-F) Flow cytometry analysis of the percent of CD8+CD3+ T cells in tumors (n=3). Data represent mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001. 'NS' to indicate non-significance. PFI: ICG-loaded PF127 hydrogel, PFIR: ropivacaine-loaded PFI hydrogel, PFIM: imiquimod-loaded PFI hydrogel, PFIRM: imiquimod and ropivacaine co-loaded PFI hydrogel.