Figure 2:

TNF and EGFR inhibition overlap in reduction of kidney cytokines after AKI, but TNF inhibition blocks leukocyte migration signals early after AKI much less efficiently than EGFR inhibition. (A) Experimental scheme: mice were subjected to bilateral IRI and treated with vehicle (V/V), etanercept (ET), erlotinib (ER) or their combination (COMB) for 2 days after AKI. (B) Serum BUN. (C) Proteomics: Venn diagram of cytokines downregulated by each treatment as compared with vehicle-treated AKI animals (n = 3/group). (D) GO term analysis of all downregulated cytokines. Outer circle denotes treatments; inner circle denotes downregulated cytokines: common between treatments (dark orange), unique (light orange). Purple curves connect identical cytokines and blue curves connect cytokines that belong to the same enriched GO term. (E) Top common GO terms of all downregulated cytokines. (F) Downregulated cytokines common to all treatments. (G) Top common GO terms of downregulated cytokines common to all treatments. (H) Enrichment analysis of transcription factors known to regulate any of the cytokines downregulated by ET, ER or COMB. NFκB family members are highlighted in red rectangle.