Table 2.
Chemical dimerizers and their applications in cell division studies.
Dimerizers | Function | Applications in cell division |
---|---|---|
AP20187 | FKBP homo-dimerization | Dimerize MPS1 for trans-activation75. |
Auxin/IAA | Degrade AID-tagged protein by recruiting ubiquitin ligases | Rapidly deplete proteins without known inhibitors82. |
CTH | Light-inducible dimerization of eDHFR and Halo enzyme | Recruit kinesin motors7 or Aurora B11 to kinetochores. |
LoKI | Inhibit kinases proximal to the SNAP-tagged protein | Inhibit pole- or kinetochore-localized pools of Aurora A and Plk187. |
Rapamycin | FKBP-FRB dimerization | Recruit Aurora B to centromeres58 or MAD1 to kinetochores72,73, test MPS1 function at different locations76,89. |
TNH | Photo-cleavable dimerizer linking eDHFR and Halo enzyme | Initiate mitotic exit by releasing MAD1 from kinetochores7. |