Macronutrient concentration in preterm breast milk on postpartum days 7 (n = 36), 14 (n = 35), 21 (n = 34), and 28 (n = 28). (A) Energy. (B) Fat. (C) Protein by race. (D) Protein by volume. At a volume of 65 mL, day 7 (P = 0.0028) and day 14 (P = 0.0066) had greater mean protein than day 28. At a volume of 150 mL, day 7 had a greater mean protein than day 21 (P = 0.0034) and day 28 (P < 0.0001), day 14 had a significantly greater mean protein than day 28 (P = 0.0019), and day 21 had a significantly greater mean protein than day 28 (P = 0.0400). At a volume of 300 mL, day 7 had a greater mean protein than day 14 (P = 0.0007), day 21 (P < 0.0001), and day 28 (P < 0.0001), and day 14 had a greater mean protein than day 28 (P = 0.0071). Among whites, day 7 had greater mean protein than day 21 (P = 0.0022) and day 28 (P = 0.0031). (E) Carbohydrates by gestational age. For those with a gestational age ≥28 wk, day 28 was lower than days 7 (P = 0.0187) and 14 (P = 0.0171). Within day 28, mean carbohydrate was lower among those with a gestational age ≥ 28 wk than among those with a gestational age < 28 wk. (F) Volume by race.