Table 2.
Sequences of the oligonucleotide primers used for quantitative reverse transcription-PCRa
| Gene | Primer sequences(5ʹ→ 3ʹ) | NCBI serial number |
|---|---|---|
| β-actin | F-CAACACAGTGCTGTCTGGTGGTAC | NM_205518.1 |
| R-CTCCTGCTTGCTGATCCACATCTG | ||
| Occludin | F-ACGGCAGCACCTACCTCAA | NM_205128.1 |
| R-GGGCGAAGAAGCAGATGAG | ||
| MUC2 | F-TCCCCTGTTGAGGGAGAACTT | XM_040673077.1 |
| R-AGTGGTTGTACCTTCGGTGC | ||
| ZO-1 | F-CTTCAGGTGTTTCTCTTCCTCCTC | XM_040680632.1 |
| R-CTGTGGTTTCATGGCTGGATC | ||
| PEPT1 | F-TACGCATACTGTCACCATCA | NM_204365.2 |
| R-TCCTGAGAACGGACTGTAAT | ||
| SGLT1 | F-GATGTGCGGATACCTGAAGC | NM_001293240.1 |
| R-AGGGATGCCAACATGACTGA | ||
| GLUT2 | F-CCGCAGAAGGTGATAGAAGC | NM_205129.1 |
| R-ATTGTCCCTGGAGGTGTT | ||
| I-FABP | F-GAAGCAATGGGCGTGAATGTGATG | NM_001007923.1 |
| R-TTCGATGTCGATGGTACGGAAGTTG | ||
| IL-1β | F-ACTGGGCATCAAGGGCTA | XM_046931582.1 |
| R-GGTAGAAGATGAAGCGGGTC | ||
| IL-6 | F-CGCCCAGAAATCCCTCCTC | NM_204628.2 |
| R-AGGCACTGAAACTCCTGGTC | ||
| TLR2 | F-GATTGTGGACAACATCATTGACTC | NM_001161650.3 |
| R-AGAGCTGCTTTCAAGTTTTCCC | ||
| TGF-β | F-TCATCACCAGGACAGCGTTA | NM_001031045.3 |
| R-TGTGATGGAGCCATTCATGT |
F Upstream primer, R Downstream primers, ZO-1 Zonula occludens-1, PEPT1 Peptide-transporter 1, SGLT1 Sodium glucose cotransporter 1, GLUT2 Glucose transporter 2, I-FABP Intestinal fatty acid binding protein, IL-1β Interleukin-1β, IL-6 Interleukin-6, TLR2 Toll like receptor 2, TGF-β Transforming growth factor-β
aThe primers were synthesized by Shanghai Shenggong Biotechnology Co., Ltd.