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. 2023 Apr 2;19(7):2021–2033. doi: 10.7150/ijbs.82744

Figure 1.

Figure 1

Osteoblastic STAT3 was closely related to exercise-mediated mechanical force in vivo. A. Experimental outline of the running-wheel exercise test. Skeletal parameters were measured in C57BL/6 mice that had access to exercise wheels for 5 weeks or not. B. H&E staining of the femurs of the control and exercise mice. C. Three-dimensional micro-CT reconstruction images of femurs from the control and exercise mice. The top panel shows trabecular bone, and the bottom panel represents cortical bone. Representative examples are shown. D. Quantitative microarchitectural parameters of micro-CT: BV/TV, Tb.Th., Tb.N., Tb.Sp. and Ct.Th. Five pairs of control and exercise mice were included in the measurement. E. Representative images of dual calcein-alizarin red S labeling of trabecular bone from the control and exercise mice acquired 5 weeks after in vivo exercise treatment and quantification of mineral apposition rate. F. Representative images of the immunofluorescence for p-STAT3 in trabecular bone from the control and exercise mice. G. Experimental outline of the tail suspension test. Skeletal parameters were measured in C57BL/6 mice that had tail-suspension treatment for 1 week or not. H. H&E staining of the femurs of the control and tail-suspension mice. I. Three-dimensional micro-CT reconstruction images of femurs from the control and tail-suspension mice. The top panel shows trabecular bone, and the bottom panel represents cortical bone. Representative examples are shown. J. Quantitative microarchitectural parameters of micro-CT: BV/TV, Tb.Th., Tb.N., Tb.Sp. and Ct.Th. Five pairs of control and tail suspension mice were included in the measurement. K. Representative images of dual calcein-alizarin red S labeling of trabecular bone from the control and tail-suspension mice acquired 1 week after tail-suspension treatment and quantification of the mineral apposition rate. L. Representative images of the immunofluorescence for p-STAT3 in trabecular bone from the control and tail suspension mice. n = 5 mice per group