Figure 2.

Osteoblastic STAT3 was activated by mechanical force in vitro. A. ALP staining of BMSCs after culture in osteogenic medium with or without mechanical force for 7 days. B. The relative mRNA levels of Runx2, Sp7, Alp, and Col1α1 in BMSCs with or without mechanical force for 7 days were quantified by qPCR. C. The expression of p-STAT3 and STAT3 in BMSCs with or without mechanical force for 8 h were analyzed by western blotting. D. Immunofluorescence staining of p-STAT3 in control and mechanical force-treated BMSCs for 8 h. E. Immunofluorescence staining of STAT3 in control and mechanical force-treated BMSCs for 8 h. Ratio of p-STAT3⁺ cells in control and mechanical force-treated BMSCs for 8 h.