Figure 5.

Pharmacological activation of STAT3 activity promoted osteoblast differentiation. A. Expression of p-STAT3 and STAT3 in BMSCs with or without colivelin for 12 h were analyzed by western blotting. B. Immunofluorescence staining of STAT3 in control and colivelin-treated BMSCs treated for 12 h. C. Immunofluorescence staining of p-STAT3 in control and colivelin-treated BMSCs treated for 12 h. The ratio of p-STAT3⁺ cells in the control and colivelin treated groups. D. ALP staining and alizarin red S staining of BMSCs after culture in osteogenic medium with or without colivelin for 7 and 14 days separately. E. The relative mRNA levels of Runx2, Sp7, Alp, and Col1α1 in BMSCs with or without colivelin for 7 days were quantified by qPCR.