Figure 6.

Pharmacological activation of STAT3 promoted osteogenesis in the absence of mechanical force in vivo. A. Experimental outline. B. Three-dimensional micro-CT reconstruction images of femurs from the control and tail-suspension mice with or without colivelin treatment. The top panel shows trabecular bone, and the bottom panel represents cortical bone. Representative examples are shown. C. Quantitative microarchitectural parameters of micro-CT: BV/TV, Tb.Th., Tb.N., Tb.Sp. and Ct.Th. Five pairs of control and exercise mice treated with or without Stat3 knockout were included in the measurement. D. Representative images of dual calcein-alizarin red S labeling of trabecular bone from the control and exercise mice treated with or without Stat3 knockout acquired 5 weeks after in vivo exercise treatment and the quantification of the mineral apposition rate. E. Representative images of TRAP staining of trabecular bone from the control and exercise mice with or without Stat3 knockout. F. Experimental outline. G. Three-dimensional micro-CT reconstruction images of femurs from the control and tail-suspension mice with or without colivelin treatment. The top panel shows trabecular bone, and the bottom panel represents cortical bone. Representative examples are shown. H. Quantitative microarchitectural parameters of micro-CT: BV/TV, Tb.Th., Tb.N., Tb.Sp. and Ct.Th. Five pairs of control and exercise mice treated with or without Stat3 knockout were included in the measurement. I. Representative images of dual calcein-alizarin red S labeling of trabecular bone from the control and exercise mice treated with or without Stat3 knockout acquired 5 weeks after in vivo exercise treatment, and the quantification of the mineral apposition rate. n = 5 mice per group.