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. 2022 Oct 14;25(5):841–853. doi: 10.1093/neuonc/noac238

Figure 1.

Figure 1.

USP36 is a novel mediator of ALKBH5. (A) The ALKBH5 expressions were detected in GSC17 cells treated with cycloheximide (CHX) or MG132 for the indicated time. (B) The ALKBH5 expressions were detected in GSC23 cells treated with CHX or MG132 for the indicated concentration. (C) LN229 cells were transfected with different deubiquitinases plasmids. The band intensity of ALKBH5 was quantified and normalized to the internal control (data are mean ± SD for triplicate biological replicates; Student t-test; *P < .05; **P < .01). (D) 293T cells were transfected with indicated plasmids. Cells were treated with 25 μM MG132 for 6 h and cell lysates were immunoprecipitated with an anti-HA antibody. The immunoprecipitates were analyzed by immunoblotting with anti-His antibodies. (E) The 293T cells were transfected with His-ALKBH5. His-ALKBH5 protein was harvested with HisPur Ni-NTA magnetic beads and subjected to mass spectrometry. The table shows that four DUB proteins with P < .05 were identified by the mass spectrometry analysis. ).