Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 14;25(5):841–853. doi: 10.1093/neuonc/noac238

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights)

PMC Copyright notice

Figure 4. — USP36 stabilizes ALKBH5 by inhibiting its ubiquitination. (A) 293T cells were transfected with indicated plasmids or specific siRNA. Cell lysates were immunoprecipitated with anti-HA antibody and then analyzed by immunoblotting. (B) 293T cells were transfected with indicated plasmids, and followed by treatment with MG132 for 6 hours before cell harvest. Cell lysates were immunoprecipitated with anti-HA antibody and then analyzed by immunoblotting using the indicated antibodies. (C) The endogenous ubiquitination of ALKBH5 was analyzed in USP36-knockout GSC17 cells after transfected with HA-Ubi. Cell lysates were immunoprecipitated with anti-HA antibody and then analyzed by immunoblotting using the indicated antibodies. (D) 293T cells were transfected with His-ALKBH5 and HA-Ubi and then treated with MG132 for 6 hours. ALKBH5 protein was purified using the magnetic bead. The ubiquitinated ALKBH5 was then incubated with 50 ng or 500 ng USP36 in a deubiquitination buffer. The resulting reactions were subjected to immunoblotting analysis.