Figure 6.

ZEB1 transcriptionally activates ARL13B expression. (A) The correlation between ARL13B and ZEB1 mRNA level in various glioma cells (A172, H4, U87-MG, U118-MG, and U251-MG) was analyzed by qPCR. (B, C) ARL13B levels were increased in ZEB1 over-expression cells. (D, E) ARL13B levels are reduced in ZEB1-depleted cells. (F) The consensus sequence of the ZEB1 binding sites (ZBS). (G) The distribution of putative ZBS candidates within −2000-bp to +500-bp of TSS of ARL13B. Luciferase reporter constructs are shown in schematic illustration. (H) ZEB1 enhanced ARL13B promoter activity. (I) ZEB1 regulates ARL13B transcription primarily through ZBS9 element. (B, D, H, I) Data are mean ± SD. P values were calculated by independent-samples t-test.