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. 2023 May 2;24(1):2203332. doi: 10.1080/15384047.2023.2203332

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 2. — FLOT1 knockdown inhibited cell migration and impeded EMT of NSCLC cells. (a) Knockdown of FLOT1 in three specific shRNA-transduced stable radioresistant A549/X and H520/X cells. (b) Representative morphological images of the indicated NSCLC cells. (c) Western blotting analysis of EMT markers (E-cadherin, N-cadherin, Vimentin, and Snail) in the indicated cells. (d) Immunofluorescence images of Vimentin in the indicated cells. (e) Wound healing assay was performed to assess the migration of A549/X and H520/X cells with different treatments. Each experiment was performed in triplicate, and the values were presented as the mean ± SD. **p < 0.01; ****p < 0.0001; not significant (n.S); as compared with sh-Control group.