Fig. 3.

Effect of virustatic or virucidal TδVD on viral particles. (a) Schematic representation of the procedure, created with BioRender.com. Concentrated A(H1N1)pdm09 stock was incubated with the drugs at a concentration ∼EC50. The solution was then fixed with glutaraldehyde 4% for 24 h at 4 °C. Viruses were negatively stained with uranyl acetate on carbon-coated copper grids, and analyzed by TEM at 29’000 times magnification. (b-d) Representative images of the virus incubated with control medium (b), virustatic 1 (c) or virucidal 3 (d). The percentage of intact and destructed viral particles are shown for each condition. The total numbers of viruses per condition were: UTR = 86 total (83 intact + 3 destructed); 1 = 75 total (73 intact + 2 destructed); 3 = 40 total (18 intact +22 destructed). N = 2 biological replicates.