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. 2023 Apr 20;16:1153870. doi: 10.3389/fnmol.2023.1153870

Figure 1.

Figure 1

Peripheral nerve injury decreased PRMT1. (A) Left: representative photomicrograph of PRMT1 (green) in the anterior cingulate cortex (ACC). Right: zoomed-in view of the boxed region in the left panel. Blue: DAPI; green: PRMT1; red arrow: DAPI; white arrow: PRMT1 and DAPI; scale bars, 200 μm (left), 50 μm (right). (B) Quantification of PRMT1 positive cells in different layers of the ACC. (C) Schematic diagram of the ligation of the common peroneal nerve (CPN) in mouse. (D) Paw withdrawal thresholds (PWTs) assessed by von Frey behavioral test after common peroneal nerve (CPN) ligation (Sham and CPN, n = 6/group, unpaired t-test, p < 0.01; **p < 0.01). (E) Prmt1 mRNA level in the ACC of Sham and CPN mice (Sham: n = 3, CPN: n = 3; unpaired t-test, p = 0.0237; *p < 0.05). (F) PRMT1 protein level in the ACC of Sham and CPN mice. Top: representative immunoblot images; bottom: statistical analysis (Sham: n = 4, CPN: n = 5; unpaired t-test, p = 0.0009; ***p < 0.001). (G) PRMT1 protein level in the auditory cortex (AuD) of Sham and CPN mice. Top: representative immunoblot images; bottom: statistical analysis (Sham: n = 3, CPN: n = 3; unpaired t-test, p = 0.039; *p < 0.01). (H) PRMT1 protein level in the lateral amygdaloid nucleus (La) of Sham and CPN mice. Top: representative immunoblot images; bottom: statistical analysis (Sham: n = 3, CPN: n = 3; unpaired t-test, p = 0.1453). Data are represented as mean ± SEM; ns, not significant.