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. 2023 Apr 20;16:1153870. doi: 10.3389/fnmol.2023.1153870

Figure 3.

Figure 3

FMRP was involved in the development of peripheral hypersensitivity induced by PRMT1 downregulation. (A) Representative examples of control (Ctrl, left) and the application of AMI-1 (right) of FMRP-GFP in HEK-293 T cells. Scale bar, 20 μm. (B) The average size of the condensed FMRP-GFP clusters in Ctrl and AMI-1 groups (Ctrl: n = 8, AMI-1: n = 7; unpaired t-test, p = 0.0338; *p < 0.05). (C) The integrated density of the condensed FMRP-GFP clusters in Ctrl and AMI-1 groups (Ctrl: n = 8, AMI-1: n = 7; unpaired t-test, p = 0.0185; *p < 0.05). (D) Representative examples of GFP (left), Ctrl (middle, transfected FMRP-GFP) and the application of AMI-1 (right, transfected FMRP-GFP) of FMRP-GFP in the cultured neurons. Scale bar, 10 μm. (E) The percentage of condensed FMRP in the FMRP-GFP clusters after applying solvent (Ctrl group, left) or AMI-1 (right). LLPS: liquid-liquid phase separation; Non-LLPS: no liquid-liquid phase separation (Chi-square test, Ctrl vs. AMI-1, *p < 0.05). (F) Confocal images of FMRP-and PRMT1-expressing cells in ACC; zoomed-in view of the boxed region in the left panel (blue, DAPI; red, PRMT1; green, FMRP; scale bars, 200 μm (left), 50 μm (right)). (G) Illustration of bilateral expression of lenti-Prmt1 shRNA in ACC of Fmr1 wild-type (WT)/knock-out (KO) mice (scale bar, 200 μm). (H) PWT tests after Prmt1 shRNA expressed in ACC of Fmr1 WT and KO mice (Two-way RM ANOVA, interaction: F(2;32) = 10.34, p < 0.01, Time: F(2;32) = 14.15, p < 0.01, WT vs. KO: F(1;32) = 47.84, p < 0.01; Baseline: WT vs. KO, p > 0.05; WT: n = 8, KO: n = 10; **p < 0.01). Data are represented as mean ± SEM; ns, not significant.