Table 2.
Real-time quantitative PCR reaction system and procedure.
| Reaction system | Volume (μL) | Program |
|---|---|---|
| 2 × PCR mix | 5 | 95°C 3 min |
| Forward primer | 0.4 | 95°C 5 s |
| Reverse primer | 0.4 | 40 cycles |
| cDNA | 1 | 60°C 15 s |
| H2O | 3.2 |
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