FIGURE 1.

Schematic biosynthesis of cultivated brown macroalgae for γ-PGA biosynthesis. [1] dry macroalgae is subjected to mechanical pre-treatment [2] the pre-treated macroalgae biomass is supplemented with sucrose and glutamic acid; followed by inoculation with a bacterial γ-PGA producer [3] the fermentation media can be supplemented with modulatory compounds, [4] isolation of γ-PGA by centrifugation occurs and [5] γ-PGA is precipitated in ethanol. [6,7] the precipitate is then re-dissolved for further purification [8] followed by freeze drying.