TABLE 1.

Summary of complex algae media, their pre-treatment and figures which display their average yields in γ-PGA.

Media name	Algae component(s)	Description
Complex Medium 1	40 g/L Commercial Laminaria digitata flakes undergone pre-treatment investigations (Cornish Seaweed Ltd., United Kingdom)	Commercial sample subjected to mechanical mortar and pestle or high shear mixing or ball mill grinding pre-treatment
Complex Medium 2	40 g/L cultivated Laminaria digitata pre-treated with high shear mixing (SAMS, Oban, United Kingdom)	Cultivated macroalgae collected and analysed for γ-PGA production at six timepoints during the year between 07.04.2021 and 01.07.2021
Complex Medium 3	40 g/L of cultivated Laminaria digitata or Alaria esculenta or Saccharina latissima pre-treated with high shear mixing (SAMS, Oban, United Kingdom)	γ-PGA yields from each algal group cultivated 07.04.2021 and 01.07.2021 were averaged to assess variation in yields during isolation at different yearly timepoints